论文标题
Lambda DNA通过等离子纳米孔通过表面增强的拉曼散射可以检测到什么？
lambda DNA through a plasmonic nanopore What can be detected by means of Surface Enhanced Raman Scattering?
论文作者
论文摘要
等离子体纳米孔中的工程电磁场可实现增强的光学检测及其在单分子测序中的使用。在这里，在厚的纳米多孔膜中制备的等离子纳米孔用于研究金属与长链双链DNA分子之间的相互作用。我们讨论了纳米多孔金属的矩阵如何与分子相互作用：i）多孔表面与DNA和ii之间的瞬时as特异性相互作用以及ii）局部场在金属纳米结构中施加的光学力。多达数十毫秒的相互作用持续时间使得可以收集高信噪比的拉曼振动，从而可以轻松地从DNA分子中读取信息。此外，为了进一步提高检测率的事件，我们测试了位于固态膜下方的聚合物多孔水凝胶。这种方法使分子扩散的放缓使检测到的相互作用数量增加了约20倍。
Engineered electromagnetic fields in plasmonic nanopores enable enhanced optical detection and their use in single molecule sequencing. Here, a plasmonic nanopore prepared in a thick nanoporous film is used to investigate the interaction between the metal and a long-chain double strand DNA molecule. We discuss how the matrix of nanoporous metal can interact with the molecule thanks to: i) transient aspecific interactions between the porous surface and DNA and ii) optical forces exerted by the localized field in a metallic nanostructure. A duration of interaction up to tens of milliseconds enables to collect high signal-to-noise Raman vibrations allowing an easy label-free reading of information from the DNA molecule. Moreover, in order to further increase the event of detection rate, we tested a polymeric porous hydrogel placed beneath the solid-state membrane. This approach enables a slowdown of the molecule diffusion, thus increasing the number of detected interactions by a factor of about 20.
