论文标题
通过质谱法分析单细胞蛋白分析
Single-cell protein analysis by mass-spectrometry
论文作者
论文摘要
人类的生理学和病理是由各种单细胞的协调相互作用引起的。但是，分析单细胞的分析限制受分析方法的敏感性和吞吐量的限制。 DNA测序最近使得对核酸的这种分析可行，但是单细胞蛋白分析仍然有限。质谱法是用于蛋白质分析的最强大方法，但其应用于单个单元格是三个主要挑战：有效地将蛋白质/肽传递到MS检测器，识别其序列并将分析缩放到数千个单元格中。这些挑战激发了相应的解决方案，包括范围设计多路复用和清洁，自动化和微型化样品制备。这些溶液协同应用，可以量化许多单个细胞的数千种蛋白质，并为进一步的进步建立坚实的基础。在这个基础的基础上，范围概念将使亚细胞细胞器和翻译后修改能够分析，而多路复用功能的增加将增加吞吐量并降低成本。
Human physiology and pathology arise from the coordinated interactions of diverse single cells. However, analyzing single cells has been limited by the low sensitivity and throughput of analytical methods. DNA sequencing has recently made such analysis feasible for nucleic acids, but single-cell protein analysis remains limited. Mass-spectrometry is the most powerful method for protein analysis, but its application to single cells faces three major challenges: Efficiently delivering proteins/peptides to MS detectors, identifying their sequences, and scaling the analysis to many thousands of single cells. These challenges have motivated corresponding solutions, including SCoPE-design multiplexing and clean, automated, and miniaturized sample preparation. Synergistically applied, these solutions enable quantifying thousands of proteins across many single cells and establish a solid foundation for further advances. Building upon this foundation, the SCoPE concept will enable analyzing subcellular organelles and post-translational modifications while increases in multiplexing capabilities will increase the throughput and decrease cost.
