论文标题
探测多尺度蛋白质力学和组装的光学镊子方法
Optical tweezers approaches for probing multiscale protein mechanics and assembly
论文作者
论文摘要
单个蛋白质构建块的多步组件是形成细胞内部和外部必需高阶结构的关键。光学镊子是一种非常适合在各种尺寸尺度上研究这些结构的力学和动力学的技术。在这次迷你审查中，我们重点介绍了使用光学镊子研究蛋白质组装和力学的实验，重点是细胞外基质蛋白胶原蛋白。这些示例表明，光学镊子如何用于研究长度尺度的力学，从单分子水平到原纤维再到蛋白质网络。我们讨论了实验设计和解释，与其他实验方式集成的机会以及光学镊子在蛋白质力学和组装中的当前问题中的应用。
Multi-step assembly of individual protein building blocks is key to the formation of essential higher-order structures inside and outside of cells. Optical tweezers is a technique well suited to investigate the mechanics and dynamics of these structures at a variety of size scales. In this mini-review, we highlight experiments that have used optical tweezers to investigate protein assembly and mechanics, with a focus on the extracellular matrix protein collagen. These examples demonstrate how optical tweezers can be used to study mechanics across length scales, ranging from the single-molecule level to fibrils to protein networks. We discuss challenges in experimental design and interpretation, opportunities for integration with other experimental modalities, and applications of optical tweezers to current questions in protein mechanics and assembly.
