论文标题
用于扩展活细胞成像的基于Arduino的低成本成像孵化器
An Arduino-based, low-cost imaging incubator for extended live cell imaging
论文作者
论文摘要
为了长时间成像活细胞，构建了一个基于Arduino的低成本成像孵化器。成像孵化器通过控制温度和CO2来使细胞保持生存，以在成像过程中维持细胞的生理条件。构建中使用的所有设备和零件都是典型的制造商型组件，以最大程度地降低成像孵化器的成本。成像孵化器允许对暴露于任何所需扰动或刺激的活细胞进行实时成像。作为系统功能的证明，将在24小时内成像，同时在成像孵化器中保持生存。
In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.
